Diagnostic PCR tests forMicrosporum audouinii, M. canisandTrichophytoninfections

Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections.

Since traditional diagnosis of dermatophyte infections is slow, we present a rapid new PCR test for detection of Trichophyton spp., Microsporum canis and M. audouinii infections. The performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human DNA control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from gu...

Assessing the Validity of Diagnostic Tests

Population structure and evolutionary origins of Microsporum canis, M. ferrugineum and M. audouinii.

The recurrent evolutionary emergence of asexual lineages within sexual zoo- and anthropophilic dermatophyte species living in animal-frequented soil is likely to be triggered by changes in ecological niche, i.e., shifts of host animal. Subsequent adaptation to the new host species is noted. Sometimes geographic isolation or intrinsic host factors like human race may also play a role in speciati...

Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by ...

Nursing: Deciphering Diagnostic Tests

Deciphering Diagnostic Tests covers over 400 laboratory tests and diagnostic procedures, organized by test type and body system. Coverage includes test purpose, normal results, abnormal results, patient preparation, and procedure and post-test care. Icons include Action Stat! for urgent interventions, Alert for important clinical facts, Do's & Don'ts for technique and intervention advice, and D...